WashU gRNA Designer for CRISPR/Cas9 Knockout



Option 1: Search for predesigned gRNA to target human or mouse genes:

Search by gene target


Option 2: gRNA design for user-provided genomic target sequence:

Custom genomic target sequences must be between 24 and 30,000 bases long.  Please submit only one sequence at a time, consisting of A, T or U, C and G.  Please note that all spaces, line breaks, and numbers within the submission will be removed, and multiple lines will be joined together to create a single sequence.

The stand-alone gRNA design tool can be downloaded here. This program runs under a Red Hat-compatible Linux system.

  • Wong N, Liu W, Wang X (2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome Biology. 16(1):218.

Click here to contact us with questions or comments about this web site. Last modified: November 03, 2015